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| No. | Name | Pos | Team | B/T | Level | Age |
| 1 | Elly De La Cruz | SS | CIN | S/R | AAA | 21 |
| 2 | Jackson Holliday | SS | BAL | L/R | High-A | 19 |
| 3 | Jordan Walker | 3B/OF | SL | R/R | AAA | 21 |
| 4 | Junior Caminero | 3B | TB | R/R | High-A | 19 |
| 5 | Jackson Chourio | OF | MIL | R/R | AA | 19 |
| 6 | Eury Perez | SP | MIA | R/R | MLB | 20 |
| 7 | James Wood | OF | WAS | L/R | High-A | 20 |
| 8 | Marcelo Mayer | SS | BOS | L/R | High-A | 20 |
| 9 | Jordan Lawlar | SS | ARI | R/R | AA | 20 |
| 10 | Jasson Dominguez | OF | NYY | S/R | AA | 20 |
| 11 | Pete Crow-Armstrong | OF | CHC | L/L | AA | 21 |
| 12 | Evan Carter | OF | TEX | L/R | AA | 20 |
| 13 | Matt McLain | SS | CIN | R/R | MLB | 23 |
| 14 | Zac Veen | OF | COL | L/R | AA | 21 |
| 15 | Jackson Merrill | SS | SD | L/R | High-A | 20 |
| 16 | Kyle Manzardo | 1B | TB | L/R | AAA | 22 |
| 17 | Royce Lewis | SS | MIN | R/R | AAA | 24 |
| 18 | Gavin Williams | SP | CLE | L/R | AAA | 23 |
| 19 | Noelvi Marte | SS/3B | CIN | R/R | AA | 21 |
| 20 | Masyn Winn | SS | SL | R/R | AAA | 21 |
| 21 | Grayson Rodriguez | SP | BAL | R/R | MLB | 23 |
| 22 | Bryce Miller | SPRP | SEA | R/R | MLB | 24 |
| 23 | Zach Neto | SS | LAA | R/R | MLB | 22 |
| 24 | Christian Encarnacion-Strand | 3B | CIN | R/R | AAA | 23 |
| 25 | Cam Collier | 3B | CIN | L/R | Low-A | 18 |